Transcriptional regulation ofmexR, the repressor ofPseudomonas aeruginosa mexAB-oprMmultidrug efflux pump
نویسندگان
چکیده
منابع مشابه
nalD encodes a second repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa.
The Pseudomonas aeruginosa nalD gene encodes a TetR family repressor with homology to the SmeT and TtgR repressors of the smeDEF and ttgABC multidrug efflux systems of Stenotrophomonas maltophilia and Pseudomonas putida, respectively. A sequence upstream of mexAB-oprM and overlapping a second promoter for this efflux system was very similar to the SmeT and TtgR operator sequences, and NalD bind...
متن کاملIn vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
There is an emerging scientific need for reliable tools for monitoring membrane protein transport. We present a methodology leading to the reconstitution of efflux pumps from the Gram-negative bacteria Pseudomonas aeruginosa in a biomimetic environment that allows for an accurate investigation of their activity of transport. Three prerequisites are fulfilled: compartmentation in a lipidic envir...
متن کاملInvestigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit
Background and Aims: Pseudomonas aeruginosa is one of the most important pathogens of nosocomial infections, especially in the ICU (Intensive Care Unit), which has resistance to a wide range of antibiotics, especially Carbapenems. Among the most important resistance mechanisms of this bacteria against carbapenems are MexAB-OprM efflux pump. Therefore, the aim of this study was to evaluate the g...
متن کاملThe mexR repressor of the mexAB-oprM multidrug efflux operon in Pseudomonas aeruginosa: characterization of mutations compromising activity.
Mutations in mexR yield a multidrug resistance phenotype in nalB mutants of Pseudomonas aeruginosa as a result of derepression of the mexAB-oprM multidrug efflux operon. MexR produced by several nalB strains carried single amino acid changes that compromised MexR stability or its ability to dimerize. Changes at residues L95 and R21, however, produced a stable MexR protein capable of dimerizatio...
متن کاملMolecular characterization of MexL, the transcriptional repressor of the mexJK multidrug efflux operon in Pseudomonas aeruginosa.
The Pseudomonas aeruginosa mexJK efflux operon is constitutively expressed in mutants with defects in the upstream mexL gene, which encodes a repressor of the TetR family. MexL and a MexL(A47D) mutant protein were purified from Escherichia coli as fusion proteins with carboxy-terminal hexahistidine tags. Native polyacrylamide gel electrophoresis and size exclusion chromatography revealed that M...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2002
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2002.tb11029.x